Cystatin E/M/CST6 Overexpression Lysate

Images

 
Western Blot: CST6 Overexpression Lysate (Adult Normal) [NBL1-09549] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CST6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cystatin E/M/CST6 Overexpression Lysate Summary

Description

Cystatin E/M/CST6 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207689

Accession#: NM_001323

Protein Tag: C-MYC/DDK
Gene
CST6

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cystatin E/M/CST6 Overexpression Lysate

  • CST6
  • cystatin 6
  • Cystatin E/M
  • cystatin M
  • cystatin M/E
  • Cystatin-6
  • cystatin-E
  • cystatin-M
  • cysteine proteinase inhibitor

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cystatin E/M/CST6 Lysate (NBL1-09549) (0)

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Bioinformatics

Gene Symbol CST6