Recombinant Human Cystathionase Protein

Product Discontinued

Recombinant Human Cystathionase Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-405 of Human CTH full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence:MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS

• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: CTH
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 70.07 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0.
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Cystathionase Protein

• cystathionase (cystathionine gamma-lyase)
• cystathionine gamma-lyase
• cysteine desulfhydrase
• EC 4.4.1
• EC 4.4.1.1
• gamma-cystathionase
• homoserine deaminase
• homoserine dehydratase
• MGC9471

Background

CTH( AAH15807, 1 a.a. - 406 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Cystathionase Recombinant Protein (H00001491-P01)(3)

Publications using H00001491-P01

• Nevoral J, Zalmanova T, Zamostna K et al. Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro. Nitric Oxide 2015-10-09 [PMID: 26456342]
• Whiteman M, Li L, Rose P et al. The effect of hydrogen sulfide donors on lipopolysaccharide-induced formation of inflammatory mediators in macrophages. Antioxid Redox Signal;12(10):1147-54. 2010-05-15 [PMID: 19769459]
• Baskar R, Li L, Moore PK. Hydrogen sulfide-induces DNA damage changes in apoptotic gene expression in human lung fibroblast cells. FASEB J;21(1):247-55. 2007-01-01 [PMID: 17116745]

Bioinformatics

• Gene Symbol: CTH