Cyr61/CCN1 Antibody

Western Blot: Cyr61/CCN1 Antibody [NB100-357] - CYR61 Antibody [NB100-357] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.

Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody [NB100-357] - CYR61 antibody was tested in MCF-7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: Cyr61/CCN1 Antibody [NB100-357] - Simple Western lane view shows a specific band for Cyr61 in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

• Reactivity: Hu, Mu(-), Rt(-)
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cyr61/CCN1 Antibody Summary

• Immunogen: A synthetic peptide made to the human CYR61 protein sequence (between residues 250-350). [UniProt# O00622]
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This CYR61 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at both approx. 37 kDa (processed form) and approx. 45 kDa (full-length form) of CYR61 protein.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 19698122). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID: 28785028).
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cyr61/CCN1 Overexpression Lysate
  Cyr61/CCN1 Knockout HeLa Cell Lysate

Reactivity Notes

Immunogen sequence has 100% homology to rat and 96% homology to mouse protein; however we have had negative feedback with the use of this antibody in mouse and rat tissue.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cyr61/CCN1 Antibody

• CCN family member 1
• CCN1
• CCN1IGF-binding protein 10
• Cyr61
• Cysteine-rich angiogenic inducer 61
• cysteine-rich, angiogenic inducer, 61
• GIG1
• GIG1cysteine-rich heparin-binding protein 61
• IBP-10
• IGFBP-10
• IGFBP10cysteine-rich, anigogenic inducer, 61
• Insulin-like growth factor-binding protein 10
• protein CYR61
• Protein GIG1

Background

CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyr61/CCN1 Antibody (NB100-357)(6)

Publications using NB100-357

• Wolf N, Yang W, Dunk CE et al. Regulation of the matricellular proteins CYR61 (CCN1) and NOV (CCN3) by hypoxia-inducible factor-1{alpha} and transforming-growth factor-{beta}3 in the human trophoblast. Endocrinology. 2010 Jun [PMID: 20237132] (IHC-Fr, Human)
• Boone, S. IFITM3 as a Potential Mechanism for Cyr61 Mediated Inhibition of Oncolytic Virotherapy. Honors Theses, College of Biological Sciences, The Ohio State University. 2011 Jun (WB, Human)
• Otani Y, Ishida J, Kurozumi K et al. PIK3R1Met326Ile germline mutation correlates with cysteine-rich protein 61 expression and poor prognosis in glioblastoma Sci Rep 2017 Aug 07 [PMID: 28785028] (IHC, Human)
• Vaithilingam V, Quayum N, Joglekar MV et al. Effect of alginate encapsulation on the cellular transcriptome of human islets. Biomaterials. 2011 Nov [PMID: 21889795] (WB, Human)
• Zhou ZQ, Cao WH, Xie JJ et al. Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma. BMC Cancer. 2009 Aug [PMID: 19698122] (WB, IHC-P, Human)
• Kurozumi K, Hardcastle J, Thakur R et al. Oncolytic HSV-1 infection of tumors induces angiogenesis and upregulates CYR61. Mol Ther. 2008 Aug [PMID: 18545226] (WB, IHC-Fr, Human, Rat)

Review for Cyr61/CCN1 Antibody (NB100-357) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: chandra kurapaty WB Human 04/29/2014

Summary

• Application: Western Blot
• Sample Tested: cell lysate, conditioned medium
• Species: Human

Blocking

• Blocking Details: 5% milk IN tbst

Primary Anitbody

• Dilution Ratio: 1:1000, o/n, 4c, 4% BSA IN tbst

Secondary Antibody

• Secondary Description: Anti-rabbit IgG, HRP-linked Antibody
• Secondary Manufacturer Cat#: 7074
• Secondary Concentration: 1:5000

Details

• Detection Notes: ECL

Bioinformatics

• Gene Symbol: CCN1
• Entrez:
  • 3491(Human)
  • 16007(Mouse)
  • 83476(Rat)
• Uniprot:
  • O00622(Human)