Cyr61/CCN1 Antibody


Western Blot: Cyr61/CCN1 Antibody [NB100-357] - CYR61 Antibody [NB100-357] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid more
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody [NB100-357] - CYR61 antibody was tested in MCF-7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Simple Western: Cyr61/CCN1 Antibody [NB100-357] - Simple Western lane view shows a specific band for Cyr61 in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Simple Western, ICC/IF
1 mg/ml

Cyr61/CCN1 Antibody Summary

A synthetic peptide made to the human CYR61 protein sequence (between residues 250-350). [UniProt# O00622]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.09% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This CYR61 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at both ~37 kDa (processed form) and ~45 kDa (full-length form) of CYR61 protein. In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa
Reviewed Applications
Read 1 Review rated 5
NB100-357 in the following application:

Read Publications using
NB100-357 in the following applications:

Reactivity Notes

Immunogen sequence has 100% homology to rat and 96% homology to mouse protein; however we have had negative feedback with the use of this antibody in mouse and rat tissue.

Alternate Names for Cyr61/CCN1 Antibody

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1


CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Mu
Applications: WB, IHC, ICC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ELISA, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IP, S-ELISA
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu
Applications: WB
Species: Hu, Mu(-), Rt(-)
Applications: WB, Simple Western, ICC/IF

Publications for Cyr61/CCN1 Antibody (NB100-357)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-357 Applications Species
Vaithilingam V, Quayum N, Joglekar MV et al. Effect of alginate encapsulation on the cellular transcriptome of human islets. Biomaterials. 2011 Nov [PMID:21889795] (WB, Human) WB Human
Wolf N, Yang W, Dunk CE et al. Regulation of the matricellular proteins CYR61 (CCN1) and NOV (CCN3) by hypoxia-inducible factor-1{alpha} and transforming-growth factor-{beta}3 in the human trophoblast. Endocrinology. 2010 Jun [PMID:20237132] (IHC-Fr, Human) IHC-Fr Human
Zhou ZQ, Cao WH, Xie JJ et al. Expression and prognostic significance of THBS1, Cyr61 and CTGF in esophageal squamous cell carcinoma. BMC Cancer. 2009 Aug [PMID:19698122] (WB, IHC-P, Human) WB, IHC-P Human
Kurozumi K, Hardcastle J, Thakur R et al. Oncolytic HSV-1 infection of tumors induces angiogenesis and upregulates CYR61. Mol Ther. 2008 Aug [PMID:18545226] (WB, IHC-Fr, Human, Rat) WB, IHC-Fr Human, Rat
Thakur R. Induction of CYR61 by Oncolytic HSV Infection of Glioma. Honors Theses, College of Biological Sciences, The Ohio State University. 2007 Jun (WB, IHC-Fr, Human, Rat) WB, IHC-Fr Human, Rat
Boone, S. IFITM3 as a Potential Mechanism for Cyr61 Mediated Inhibition of Oncolytic Virotherapy. Honors Theses, College of Biological Sciences, The Ohio State University. 2011 Jun (WB, Human) WB Human

Reviews for Cyr61/CCN1 Antibody (NB100-357) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-357:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
reviewed by:
WB Human 04/29/2014


ApplicationWestern Blot
Sample Testedcell lysate, conditioned medium


Blocking Details5% milk IN tbst

Primary Anitbody

Dilution Ratio1:1000, o/n, 4c, 4% BSA IN tbst

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7074
Secondary Concentration1:5000


Detection NotesECL

Product General Protocols

View specific protocols for Cyr61/CCN1 Antibody (NB100-357): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyr61/CCN1 Antibody (NB100-357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-357AF405
Alexa Fluor (R) 488 Labeled NB100-357AF488
Alexa Fluor (R) 647 Labeled NB100-357AF647
Alexa Fluor (R) 700 Labeled NB100-357AF700
Biotin Labeled NB100-357B
DyLight 405 Labeled NB100-357V
DyLight 488 Labeled NB100-357G
DyLight 550 Labeled NB100-357R
DyLight 650 Labeled NB100-357C
FITC Labeled NB100-357F
HRP Labeled NB100-357H

Additional Cyr61/CCN1 Antibody Products

Related Products by Gene

Bioinformatics Tool for Cyr61/CCN1 Antibody (NB100-357)

Discover related pathways, diseases and genes to Cyr61/CCN1 Antibody (NB100-357). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cyr61/CCN1 Antibody (NB100-357)

Discover more about diseases related to Cyr61/CCN1 Antibody (NB100-357).

Pathways for Cyr61/CCN1 Antibody (NB100-357)

View related products by pathway.

PTMs for Cyr61/CCN1 Antibody (NB100-357)

Learn more about PTMs related to Cyr61/CCN1 Antibody (NB100-357).

Blogs on Cyr61/CCN1

There are no specific blogs for Cyr61/CCN1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol CYR61

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-357 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought