Cyr61/CCN1 Antibody

Images

 
Western Blot: Cyr61/CCN1 Antibody [NB100-357] - CYR61 Antibody [NB100-357] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid ...read more
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody [NB100-357] - CYR61 antibody was tested in MCF-7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Simple Western: Cyr61/CCN1 Antibody [NB100-357] - Simple Western lane view shows a specific band for Cyr61 in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Cyr61/CCN1 Antibody Summary

Immunogen
A synthetic peptide made to the human CYR61 protein sequence (between residues 250-350). [UniProt# O00622]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYR61
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
Application Notes
This CYR61 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at both approx. 37 kDa (processed form) and approx. 45 kDa (full-length form) of CYR61 protein.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Cyr61/CCN1 Lysate (NBL1-09705)
Reviewed Applications
Read 1 Review rated 5
using
NB100-357 in the following applications:

Publications
Read Publications using
NB100-357 in the following applications:

Reactivity Notes

Immunogen sequence has 100% homology to rat and 96% homology to mouse protein; however we have had negative feedback with the use of this antibody in mouse and rat tissue.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyr61/CCN1 Antibody

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cyr61
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1

Background

CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyr61/CCN1 Antibody (NB100-357)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.


Filter By Application
IHC-Fr
(3)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(6)
Rat
(2)
All Species

Review for Cyr61/CCN1 Antibody (NB100-357) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-357:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
chandra kurapaty
WB Human 04/29/2014
View

Summary

ApplicationWestern Blot
Sample Testedcell lysate, conditioned medium
SpeciesHuman

Blocking

Blocking Details5% milk IN tbst

Primary Anitbody

Dilution Ratio1:1000, o/n, 4c, 4% BSA IN tbst

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7074
Secondary Concentration1:5000

Details

Detection NotesECL

Product General Protocols

View specific protocols for Cyr61/CCN1 Antibody (NB100-357): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cyr61/CCN1 Antibody (NB100-357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-357AF405
Alexa Fluor 488 Labeled NB100-357AF488
Alexa Fluor 647 Labeled NB100-357AF647
Alexa Fluor 700 Labeled NB100-357AF700
Biotin Labeled NB100-357B
DyLight 405 Labeled NB100-357V
DyLight 488 Labeled NB100-357G
DyLight 550 Labeled NB100-357R
DyLight 650 Labeled NB100-357C
FITC Labeled NB100-357F
HRP Labeled NB100-357H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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chandra kurapaty
04/29/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CYR61
Entrez
Uniprot