Cyr61/CCN1 Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line and SK‑BR‑3 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 52 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Cyr61/CCN1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cyr61/CCN1
  Ala22-Asp381
  Accession # O00622
• Specificity: Detects human Cyr61/CCN1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CYR61

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • ELISA
• Control:

  Cyr61/CCN1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyr61/CCN1 Antibody [Unconjugated]

• CCN family member 1
• CCN1
• CCN1IGF-binding protein 10
• Cyr61
• Cysteine-rich angiogenic inducer 61
• cysteine-rich, angiogenic inducer, 61
• GIG1
• GIG1cysteine-rich heparin-binding protein 61
• IBP-10
• IGFBP-10
• IGFBP10cysteine-rich, anigogenic inducer, 61
• Insulin-like growth factor-binding protein 10
• protein CYR61
• Protein GIG1

Background

Cysteine-rich angiogenic inducer 61 (Cyr61), also known as CCN1, is a 40‑45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4‑6). Plasmin cleavage of
ECM‑bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8‑12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
2. Jay, P. et al. (1997) Oncogene 14:1753.
3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
5. Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248.
6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
10. Xie, D. et al. (2004) Cancer Res. 64:1987.
11. Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594.
12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
16. Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYR61
• Uniprot:
  • O00622(Human)