CYP24A1 Antibody

Western Blot: CYP24A1 Antibody [NBP1-52111] - Analysis of CYP24A1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-52111 at 0.1 ug/m. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CYP24A1 Antibody [NBP1-52111] - 3.75ug/ml staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CYP24A1 Antibody Summary

• Immunogen: Peptide with sequence C-RKYDIQATDN corresponding to internal region according to NP_000773.2, NP_001122387.1.
• Epitope: C-RKYDIQATDN
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYP24A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: Western blot: Approx 50kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2kDa according to NP_001122387.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Skin.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYP24A1 Antibody

• 24-OHase
• 25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• CP241
• CYP24
• CYP241,25-alphadihydroxyvitamin D3 24-hydroxylase
• CYP24A1
• Cytochrome P450 24A1
• cytochrome P450, family 24, subfamily A, polypeptide 1,24-OHase
• cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
• Cytochrome P450-CC24
• EC 1.14.13.n4
• EC 1.14.15.16
• exo-mitochondrial protein
• MGC126273
• MGC126274
• P450-CC24
• vitamin D 24-hydroxylase
• Vitamin D(3) 24-hydroxylase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYP24A1 Antibody (NBP1-52111)(1)

Publication using NBP1-52111

• Shen H, Bielak LF, Ferguson JF et al. Association of the vitamin D metabolism gene CYP24A1 with coronary artery calcification. Arterioscler Thromb Vasc Biol 2010-12-01 [PMID: 20847308]

Bioinformatics

• Gene Symbol: CYP24A1
• Entrez:
  • 1591(Human)
• Uniprot:
  • NP_000773.2(Human)