Cyclophilin 40 Antibody

Western Blot: Cyclophilin 40 Antibody [NB120-3562] - Analysis of rat spleen extracts.

Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Hepatocarcinoma tissue.

Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in A431 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.

Product Discontinued

Cyclophilin 40 Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.
• Localization: Cytoplasmic
• Specificity: Detects cyclophilin 40 (CyP 40) from human and rat tissues and cells. This does not cross-react with CyPA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPID
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Simple Western
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 40 kDa protein representing CyP 40 from rat brain extract.
  See Simple Western Antibody Database for Simple Western validation: Separated by Size-All; matrix was 12-180.
• Control:

  Cyclophilin 40 Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 8662874,). Chicken (9672247). Rabbit (8662874). Primate ( 8524246). Bovine (1544925).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Alternate Names for Cyclophilin 40 Antibody

• 40 kDa peptidyl-prolyl cis-trans isomerase D
• 40 kDa peptidyl-prolyl cis-trans isomerase
• cyclophilin D
• cyclophilin-40
• Cyclophilin-related protein
• CYP40
• CYP-40cyclophilin 40
• CYPD
• EC 5.2.1.8
• MGC33096
• peptidyl-prolyl cis-trans isomerase D
• peptidylprolyl isomerase D (cyclophilin D)
• peptidylprolyl isomerase D
• PPIase D
• Rotamase D

Background

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin 40 Antibody (NB120-3562)(1)

Publication using NB120-3562

• Tombo N, Imam Aliagan AD, Feng Y et al. Cardiac Ischemia/Reperfusion Stress Reduces Inner Mitochondrial Membrane Protein (Mitofilin) Levels During Early Reperfusion Free Radic. Biol. Med. 2020-07-26 [PMID: 32726689] (WB, IP, Human)

Bioinformatics

• Gene Symbol: PPID
• Entrez:
  • 5481(Human)
  • 361967(Rat)