Cyclophilin 40 Antibody

Images

 
Western Blot: Cyclophilin 40 Antibody [NB120-3562] - Analysis of rat spleen extracts.
Immunocytochemistry/ Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were ...read more
Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Hepatocarcinoma tissue.
Immunocytochemistry/ Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in A431 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were ...read more
Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, RbSpecies Glossary
Applications WB, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Cyclophilin 40 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C A(356) Q K D K E K A V Y A K M F A(370) of human Cyp-40.
Localization
Cytoplasmic
Specificity
Detects cyclophilin 40 (CyP 40) from human and rat tissues and cells. This does not cross-react with CyPA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPID
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:1000
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 40 kDa protein representing CyP 40 from rat brain extract.
Control
Cyclophilin 40 Overexpression Lysate
Cyclophilin 40 Knockout HeLa Cell Lysate
Publications
Read Publication using
NB120-3562 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 8662874,). Chicken (9672247). Rabbit (8662874). Primate ( 8524246). Bovine (1544925).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for Cyclophilin 40 Antibody

  • 40 kDa peptidyl-prolyl cis-trans isomerase D
  • 40 kDa peptidyl-prolyl cis-trans isomerase
  • cyclophilin D
  • cyclophilin-40
  • Cyclophilin-related protein
  • CYP40
  • CYP-40cyclophilin 40
  • CYPD
  • EC 5.2.1.8
  • MGC33096
  • peptidyl-prolyl cis-trans isomerase D
  • peptidylprolyl isomerase D (cyclophilin D)
  • peptidylprolyl isomerase D
  • PPIase D
  • Rotamase D

Background

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Cyclophilin 40 Antibody (NB120-3562)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Human
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPID
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