Cyclin T1 Antibody

Immunohistochemistry-Paraffin: Cyclin T1 Antibody [NBP1-02800] - Staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

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Cyclin T1 Antibody Summary

• Immunogen: Cyclin T1 peptide corresponding to the internal region of the human protein, conjugated with KLH.
• Specificity: Antiserum will specifically react with an 85 kDa Cyclin T1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin T1 from other species may also occur.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCNT1
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:5000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: IHC: NBP1-02800 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 75 mg/ml
• Purity: Delipidation and Defibrination

Alternate Names for Cyclin T1 Antibody

• CCNT
• CCNT1
• CCNTcyclin-T
• CDK9-associated C-type protein
• cyclin C-related protein
• cyclin T1
• cyclin T1b
• Cyclin-T
• cyclin-T1
• CYCT1
• HIVE1
• human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
• Human immunodeficiency virus-1 expression
• subunit of positive elongation transcription factor b

Background

Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNT1
• Entrez:
  • 904(Human)
• Uniprot:
  • O60563(Human)