CYBB/NOX2 Antibody

Images

 
Western Blot: CYBB/NOX2 Antibody [NBP1-51928] - Analysis of CYBB/NOX2 in Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer) using NBP1-51928 at 0.03ug/ml. Primary incubation was 1 hour. Detected ...read more
Immunohistochemistry-Paraffin: CYBB/NOX2 Antibody [NBP1-51928] - Analysis in Human Spleen shows staining of select cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

CYBB/NOX2 Antibody Summary

Immunogen
Peptide with sequence C-SYLNFARKRIKNP corresponding to internal region according to NP_000388.2.
Epitope
C-SYLNFARKRIKNP
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 85Da band observed in human peripheral blood mononucleocytes lysates is reported in scientific literature (PMID: 3600769). IHC-P: Human spleen shows staining of select cells.
Publications
Read Publication using NBP1-51928.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYBB/NOX2 Antibody

  • CGD
  • CGD91-phox
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.6.3
  • GP91-1
  • GP91PHOX
  • GP91-PHOX
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Background

NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYBB/NOX2 Antibody (NBP1-51928)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CYBB/NOX2 Antibody (NBP1-51928). (Showing 1 - 1 of 1 FAQ).

  1. We would like to use the CYBB (NOX2) antibody for Western blotting on rat heart samples. Please, suggest us an alternative solution, if you have any!
    • I have taken a look for an alternative antibody, and we do have NBP1-51928 available. It has been validated for use in Western Blot however we have not yet tested this with Rat. I have carried out a BLAST on a protein database called UniProt and I have found that the percentage homology between the human CYBB and rat CYBB is 92.0%. If you would like to test this species you may find our Innovators Reward Program of interest, where we reward customers for trying our antibodies in novel species and applications.

Secondary Antibodies

 

Isotype Controls

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Diseases for CYBB/NOX2 Antibody (NBP1-51928)

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Bioinformatics

Gene Symbol CYBB
Entrez
Uniprot