CYB5R4 Antibody

Western Blot: CYB5R4 Antibody [NBP2-27496] - NBP2-27496 (0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CYB5R4 Antibody Summary

• Immunogen: Peptide with sequence RCQLEKLALREKRFD corresponding to internal region according to NP_077157.2.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CYB5R4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in rat spleen lysates (calculated MW of 55 kDa band according to NP_596918.2 and 58.8 kDa band according to Q68EJ0-1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYB5R4 Antibody

• b5+b5R
• cb5/cb5R
• cytochrome b5 reductase 4
• cytochrome b-type NAD(P)H oxidoreductase
• dJ676J13.1
• EC 1.6.2.2
• Flavohemoprotein b5/b5R
• flavohemoprotein b5+b5R
• NADPH cytochrome B5 oxidoreductase
• NCB5ORN-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containingprotein
• RP4-676J13.1

Background

NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase (DIA1; MIM 250800).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYB5R4 Antibody (NBP2-27496)(1)

Publication using NBP2-27496

• Xu M, Wang W, Frontera JR et al. Ncb5or deficiency increases fatty acid catabolism and oxidative stress. J Biol Chem 2011-04-01 [PMID: 21300801]

Bioinformatics

• Gene Symbol: CYB5R4
• Entrez:
  • 266690(Mouse)
• Uniprot:
  • NP_077157.2(Human)