Recombinant Human CYB5R3 His Protein

SDS-Page: Recombinant Human CYB5R3 Protein [NBP1-78879] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human CYB5R3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-301 of Human CYB5R3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CYB5R3
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human CYB5R3 His Protein

• B5RNADH-cytochrome b5 reductase 3
• cytochrome b5 reductase 3
• Cytochrome b5 reductase
• DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
• diaphorase (NADH) (cytochrome b-5 reductase)
• diaphorase-1
• EC 1.6.2.2
• NADH-cytochrome b5 reductase 3 soluble form

Background

CYB5R3 is a cytochrome b5 reductasecytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Mutations in this gene cause methemoglobinemias. Recombinant human CYB5R3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CYB5R3 Recombinant Protein (NBP1-78879)(1)

Publication using NBP1-78879

• Kiger L, Keith J, Freiwan A et al. Redox-Regulation of alpha-Globin in Vascular Physiology Antioxidants (Basel, Switzerland) 2022-01-14 [PMID: 35052663]

Bioinformatics

• Gene Symbol: CYB5R3