CYB5R3 Antibody

Product Discontinued

CYB5R3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human CYB5R3.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYB5R3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of CYB5R3 (I169) pAb in extracts from hela cells.
  Immunohistochemistry (IHC) analyzes of CYB5R3 (I169) pAb in paraffin-embedded human placenta tissue.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CYB5R3 Antibody

• B5RNADH-cytochrome b5 reductase 3
• cytochrome b5 reductase 3
• Cytochrome b5 reductase
• DIA1NADH-cytochrome b5 reductase 3 membrane-bound form
• diaphorase (NADH) (cytochrome b-5 reductase)
• diaphorase-1
• EC 1.6.2.2
• NADH-cytochrome b5 reductase 3 soluble form

Background

CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane,while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction. A serine residue at position 117 seems to only be found in persons of African origin. The allele frequency is 0.23 in African Americans. It is not found in Caucasians,Asians, Indo-Aryans or Arabs. This difference seems to have no effect on the enzyme activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYB5R3
• Entrez:
  • 1727(Human)
• Uniprot:
  • P00387(Human)