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CXCR7/RDC-1 Antibody (896032R) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with (A) rat CXCR7/RDC-1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB8399R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with (A) rat CXCR7/RDC-1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB8399R) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
896032R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8399RU-100UG
Alexa Fluor 405
FAB8399RV-100UG
Alexa Fluor 488
FAB8399RG-100UG
Alexa Fluor 594
FAB8399RT-100UG
Alexa Fluor 647
FAB8399RR-100UG
Alexa Fluor 700
FAB8399RN-100UG
Alexa Fluor 750
FAB8399RS-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8399R-SP
Carrier Free
FAB8399RR-100UG
Carrier Free
FAB8399RV-100UG
Carrier Free
FAB8399RG-100UG
Carrier Free
FAB8399RT-100UG
Carrier Free
FAB8399RS-100UG
Carrier Free
FAB8399RN-100UG
Carrier Free
FAB8399RU-100UG
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CXCR7/RDC-1 Antibody (896032R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
NS0 mouse myeloma cell line transfected with rat CXCR7/RDC-1
Met1-Lys362
Accession # NP_445804
Specificity
Detects rat CXCR7/RDC-1 in ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (896032R) [Unconjugated]

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Background

CXCR7 (CXC Chemokine Receptor 7), also known as GPRN1, RDC-1 and Chemokine Orphan Receptor 1, is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF-1, and appears to involve beta -Arrestin 2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF-1, and I-TAC may actually block some SDF-1-mediated migration activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunohistochemistry-Paraffin: CRLR Antibody [NLS6731] - Anti-CALCRL / CGRP Receptor antibody IHC of human Lung, Non-Small Cell Carcinoma. Tissue after heat-induced antigen retrieval.
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Antibody - Pan Specif
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CXCR7/RDC-1
Antibody (896032R) [U ...
MAB8399R
CXCR7/RDC-1 Antibody (896032R) [Unconjugated]
Species: Rt
Applications: Flow, CyTOF-ready

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB8399RU
Alexa Fluor 405 FAB8399RV
Alexa Fluor 488 FAB8399RG
Alexa Fluor 594 FAB8399RT
Alexa Fluor 647 FAB8399RR
Alexa Fluor 700 FAB8399RN
Alexa Fluor 750 FAB8399RS
Learn about Custom Antibody Labeling

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