CXCR7/RDC-1 Antibody (734110) [Unconjugated]

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB7167, filled histo­gram) or isotype control antibody rat IgM (open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgM Secondary Antibody.

HEK293 human embryonic kidney cell line transfected with eGFP and Mouse CXCR7/RDC-1 were stained with Rat Anti-Mouse CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB7167) followed by Allophycocyanin-conjugated-Anti-Rat IgM secondary antibody. Quadrant markers were set based on control antibody staining.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 734110
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR7/RDC-1 Antibody (734110) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCR7/RDC-1 extracellular N-terminus and loops
  Met1-Leu47, Ser103-Lys118, Lys184-Glu213, Leu274-Ala296
  Accession # P56485
• Specificity: Detects mouse CXCR7/RDC-1 in direct ELISAs.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Gene: CXCR7
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (734110) [Unconjugated]

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC-1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF1, and I-TAC may actually block some SDF1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over the collective extracellular domains (aa 1-47, 103-118, 184-213 and 274-296), mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (MAB7167)(2)

Publications using MAB7167

• C Shan, Y Ma MicroRNA-126/stromal cell-derived factor 1/C-X-C chemokine receptor type 7 signaling pathway promotes post-stroke angiogenesis of endothelial progenitor cell transplantation Mol Med Rep, 2018-01-29;0(0):. 1/29/2018 [PMID: 29393458]
• Chen Q, Zhang M, Li Y, Xu D, Wang Y, Song A, Zhu B, Huang Y, Zheng J CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4. Stem Cells, 2015-05-13;33(8):2574-85. 2015-05-13 [PMID: 25833331] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CXCR7
• Uniprot:
  • P56485()