CXCR7/RDC-1 Antibody (358426) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: Flow
• Clone: 358426
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

CXCR7/RDC-1 Antibody (358426) [Alexa Fluor® 405] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
  Met1-Lys362 (Gly131Ser)
  Accession # AAA62370
• Specificity: Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR7

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (358426) [Alexa Fluor® 405]

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCR7
• Uniprot:
  • AAA62370()