CXCR1/IL-8RA Antibody (42705R) [Unconjugated]

Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330R). The ND50 is typically 0.4-2.0 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 42705R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCR1/IL-8RA Antibody (42705R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
  Met1-Leu350
  Accession # AAA59159
• Specificity: Detects human CXCR1/IL-8 RA transfectants but not the parental cell line.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 0.5-25 ug/mL
  • Neutralization 0.4-2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR1/IL-8RA Antibody (42705R) [Unconjugated]

• CD128
• CD181 antigen
• CD181
• CDw128aC-C
• chemokine (C-X-C motif) receptor 1
• CKR-1
• CMKAR1
• C-X-C chemokine receptor type 1
• CXCR1
• CXC-R1
• CXCR-1
• High affinity interleukin-8 receptor A
• IL-8 RA
• IL-8 receptor type 1
• IL-8R A
• IL8R1
• IL8RA
• IL8RAC-C-CKR-1
• IL8RBA
• interleukin 8 receptor, alpha
• interleukin-8 receptor type 1
• interleukin-8 receptor type A

Background

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAA59159()