CXCL5/ENA-78 Antibody (33170) [Unconjugated]

F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." > F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." title="Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." />

Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL5/ ENA-78 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654). The ND₅₀is typically 0.65-5 µg/mL." > 254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL5/ ENA-78 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654). The ND₅₀is typically 0.65-5 µg/mL." title="Recombinant Human CXCL5/ENA-78 (254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL5/ ENA-78 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654). The ND₅₀is typically 0.65-5 µg/mL." />

Recombinant Human CXCL5/ENA-78 (<a class=

254-XB) was serially diluted and captured by Mouse Anti-Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 254-XB) was serially diluted and captured by Mouse Anti-Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human CXCL5/ENA‑78 (Catalog # 254-XB) was serially diluted and captured by Mouse Anti-Human CXCL5/ENA‑78 Monoclonal Antibody (Catalog # MAB654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL5/ENA‑78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human CXCL5/ENA‑78 (Catalog # <a class=

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, ELISA(Cap), Flow
• Clone: 33170
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL5/ENA-78 Antibody (33170) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL5/ENA-78
  Ala37-Asn114
  Accession # P42830
• Specificity: Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL5
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.65-5 ug/mL
• Application Notes: ELISA Detection: Human CXCL5/ENA-78 Biotinylated Antibody (Catalog number BAF254)
  Standard: Recombinant Human CXCL5/ENA-78 (Catalog number 254-XB)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL5/ENA-78 Antibody (33170) [Unconjugated]

• chemokine (C-C motif) ligand 5
• C-X-C motif chemokine 5
• CXCL5
• CXCL5/ENA-78
• ENA78
• ENA-78
• ENA-78(1-78)
• Epithelial-derived neutrophil-activating protein 78
• Neutrophil-activating peptide ENA-78
• neutrophil-activating protein 78
• SCYB5ENA78

Background

CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL5/ENA-78 Antibody (MAB654)(2)

Publications using MAB654

• Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment Cell reports 2023-12-30 [PMID: 38232734] (Mass Cytometry, Mouse)
• Edwards MR, Johnson MW, Johnston SL Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells. Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24. 2006-01-19 [PMID: 16424382] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CXCL5
• Uniprot:
  • P42830()