CXCL4/PF4 Antibody (170138) [Phycoerythrin]

Images

 
Human immature dendritic cells were stained with Mouse Anti-Human CXCL4/PF4 PE‑conjugated Monoclonal Antibody (Catalog # IC7952P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
170138
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

CXCL4/PF4 Antibody (170138) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human CXCL4/PF4
Glu32-Ser101
Accession # P02776.2
Specificity
Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PF4
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC7952P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL4/PF4 Antibody (170138) [Phycoerythrin]

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A

Background

CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Scheuerer, B. et al. (2000) Blood 95:1158.
  3. Perollet, C. et al. (1998) Blood 91:3289.
  4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
  5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
  6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL4/PF4 Antibody (IC7952P)(1)

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Bioinformatics

Gene Symbol PF4
Uniprot