Western blot shows lysate of mouse platelets. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog ...read more
E. coli-derived recombinant mouse CXCL4/PF4 Val30-Ser105 Accession # Q9Z126
Detects mouse CXCL4/PF4 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react or interfere recombinant human (rh) CXCL/PF4, recombinant mouse (rm) CXCL9/MIG, rmGM-CSF, rmCXCL11/I-TAC, rhCXCL8/IL-8, rmIL-5, or rhCXCL10/IP-10. In Western blots, no cross-reactivity was observed with rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta,
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CXCL4/PF4 Antibody (140910) [Unconjugated]
chemokine (C-X-C motif) ligand 4
C-X-C motif chemokine 4
platelet factor 4
CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
Poncz, M. et al. (1987) Blood 69:219.
Scheuerer, B. et al. (2000) Blood 95:1158.
Perollet, C. et al. (1998) Blood 91:3289.
Petersen, F. et al. (1998) J. Immunol. 161:4347.
Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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