CXCL4/PF4 Antibody (140910) [Unconjugated]

Western blot shows lysate of mouse platelets. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL4/PF4 Monoclonal Antibody (Catalog # MAB595) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 140910
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CXCL4/PF4 Antibody (140910) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL4/PF4
  Val30-Ser105
  Accession # Q9Z126
• Specificity: Detects mouse CXCL4/PF4 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react or interfere recombinant human (rh) CXCL/PF4, recombinant mouse (rm) CXCL9/MIG, rmGM-CSF, rmCXCL11/I-TAC, rhCXCL8/IL-8, rmIL-5, or rhCXCL10/IP-10. In Western blots, no cross-reactivity was observed with rhGCP-2, rmGCP-2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhMIP-1 alpha , rmMIP-1 alpha , rmMIP-1 beta , rhMIP-1δ, rmMIP-1 gamma , rmMIP-2, rhMIP-3 alpha , rmMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , or rmMIP-3 beta ,
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: PF4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 2 ug/mL
• Application Notes: ELISA Detection: Mouse CXCL4/PF4 Biotinylated Antibody (Catalog number BAF595)
  Standard: Recombinant Mouse CXCL4/PF4 (Catalog number 595-P4)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL4/PF4 Antibody (140910) [Unconjugated]

• chemokine (C-X-C motif) ligand 4
• C-X-C motif chemokine 4
• CXCL4
• CXCL4iroplact
• Iroplact
• MGC138298
• Oncostatin-A
• PF4
• PF-4
• platelet factor 4
• SCYB4oncostatin-A

Background

CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

1. Poncz, M. et al. (1987) Blood 69:219.
2. Scheuerer, B. et al. (2000) Blood 95:1158.
3. Perollet, C. et al. (1998) Blood 91:3289.
4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL4/PF4 Antibody (MAB595)(6)

Publications using MAB595

• X Zhang, T Takeuchi, A Takeda, H Mochizuki, Y Nagai Comparison of serum and plasma as a source of blood extracellular vesicles: Increased levels of platelet-derived particles in serum extracellular vesicle fractions alter content profiles from plasma extracellular vesicle fractions PLoS ONE, 2022-06-24;17(6):e0270634. 2022-06-24 [PMID: 35749554] (Western Blot, Mouse)
• S Wichaiyo, S Lax, SJ Montague, Z Li, B Grygielska, JA Pike, EJ Haining, A Brill, SP Watson, J Rayes Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice Haematologica, 2019-02-07;0(0):. 2019-02-07 [PMID: 30733265] (IHC-P, Mouse)
• T Barwari, S Eminaga, U Mayr, R Lu, PC Armstrong, MV Chan, M Sahraei, M Fernández-, T Moreau, J Barallobre, M Lynch, X Yin, C Schulte, F Baig, R Pechlaner, SR Langley, A Zampetaki, P Santer, M Weger, R Plasenzott, M Schosserer, J Grillari, S Kiechl, J Willeit, AM Shah, C Ghevaert, TD Warner, C Fernández-, Y Suárez, M Mayr Inhibition of profibrotic microRNA-21 affects platelets and their releasate JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385722] (IHC, Mouse)
• Lindsey ML, Jung M, Yabluchanskiy A et al. Exogenous CXCL4 Infusion Inhibits Macrophage Phagocytosis by Limiting CD36 Signaling to Enhance Post-myocardial Infarction Cardiac Dilation and Mortality. Cardiovasc. Res. 8/29/2018 [PMID: 30169632]
• E Tkaczynski, A Arulselvan, J Tkaczynski, S Avery, L Xiao, B Torok-Stor, K Abrams, NV Rao, G Johnson, TP Kennedy, M Poncz, MP Lambert 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia Blood Adv, 2018-04-10;2(7):754-761. 2018-04-10 [PMID: 29599195] (IHC-P, Mouse)
• Ren Q, Wimmer C, Chicka MC Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis. Blood, 2010-04-30;116(6):869-77. 2010-04-30 [PMID: 20435885] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PF4
• Uniprot:
  • Q9Z126()