CXCL17/VCC-1 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL17/VCC-1 Antibody Summary

• Immunogen: E. coli-derived recombinant human DMC
  Leu24-Leu119
  Accession # Q6UXB2
• Specificity: Detects human DMC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse DMC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CXCL17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL17/VCC-1 Antibody

• Chemokine (C-X-C Motif) Ligand 17
• C-X-C Motif Chemokine 17
• CXCL17
• Dcip1
• Dendritic Cell And Monocyte Chemokine-Like Protein
• DMC
• UNQ473
• VCC1
• VCC-1
• VEGF Coregulated Chemokine 1
• VEGF Co-Regulated Chemokine 1

Background

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation site. Cleavage of a 23 amino acid (aa) signal sequence yields the mature 96 aa human DMC. DMC is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS-activated peripheral blood monocytes and dendritic cells (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC, plus two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show a correlated expression pattern with VEGF in primary lung, breast and esophageal tumors (2). DMC is, therefore, suggested to play a role in tumor angiogenesis. Mature human DMC shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat DMC, respectively.

1. Pisabarro, M.T. et al. (2006) J. Immunol. 176:2069.
2. Weinstein, E.J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL17/VCC-1 Antibody (AF4207)(2)

Publications using AF4207

• Lin-pei Wang, Jing Lin, Xiao-qiu Ma, Dong-yao Xu, Chun-feng Shi, Wei Wang et al. RETRACTED ARTICLE: Exosomal DLX6-AS1 from hepatocellular carcinoma cells induces M2 macrophage polarization to promote migration and invasion in hepatocellular carcinoma through microRNA-15a-5p/CXCL17 axis Journal of Experimental & Clinical Cancer Research 12/1/2021 [PMID: 34039401]
• Zhou Z, Lu X, Zhu P, Zhu W, Mu X, Qu R, Li M VCC-1 over-expression inhibits cisplatin-induced apoptosis in HepG2 cells. Biochem. Biophys. Res. Commun., 2012-03-07;420(2):336-42. 2012-03-07 [PMID: 22425983] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: CXCL17
• Uniprot:
  • Q6UXB2()