CXCL17/VCC-1 Antibody (422208) [Unconjugated]

A549 human lung carcinoma cell line was stained with Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

CXCL17/VCC-1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 422208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL17/VCC-1 Antibody (422208) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL17/VCC‑1
  Leu24-Leu119
  Accession # Q6UXB2
• Specificity: Detects human CXCL17/VCC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CXCL17 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL17
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL17/VCC-1 Antibody (422208) [Unconjugated]

• Chemokine (C-X-C Motif) Ligand 17
• C-X-C Motif Chemokine 17
• CXCL17
• Dcip1
• Dendritic Cell And Monocyte Chemokine-Like Protein
• DMC
• UNQ473
• VCC1
• VCC-1
• VEGF Coregulated Chemokine 1
• VEGF Co-Regulated Chemokine 1

Background

CXCL17, also known as dendritic cell and monocyte chemokine-like protein (DMC) and VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation site. Cleavage of a 23 amino acid (aa) signal sequence yields the mature 96 aa human CXCL17. CXCL17 is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS-activated peripheral blood monocytes and dendritic cells (1). CXCL17 expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). CXCL17, plus two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show a correlated expression pattern with VEGF in primary lung, breast and esophageal tumors (2). CXCL17 is, therefore, suggested to play a role in tumor angiogenesis. Mature human CXCL17 shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat CXCL17, respectively.

1. Pisabarro, M.T. et al. (2006) J. Immunol. 176:2069.
2. Weinstein, E.J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL17/VCC-1 Antibody (MAB4207)(7)

Publications using MAB4207

• Y Li, A Liu, S Liu, L Yan, Y Yuan, Q Xu Involvement of CXCL17 and GPR35 in Gastric Cancer Initiation and Progression International Journal of Molecular Sciences, 2022-12-29;24(1):. 2022-12-29 [PMID: 36614059] (IHC, Western Blot, Human)
• Jose Alberto Choreño-Parra, Luis Armando Jiménez-Álvarez, Gustavo Ramírez-Martínez, Montserrat Sandoval-Vega, Citlaltepetl Salinas-Lara, Carlos Sánchez-Garibay et al. CXCL17 Is a Specific Diagnostic Biomarker for Severe Pandemic Influenza A(H1N1) That Predicts Poor Clinical Outcome Frontiers in Immunology 2/26/2021 [PMID: 33717172]
• H Yao, Y Lv, X Bai, Z Yu, X Liu Prognostic value of CXCL17 and CXCR8 expression in patients with colon cancer Oncol Lett, 2020-07-07;20(3):2711-2720. 2020-07-07 [PMID: 32782587] (Immunohistochemistry, Human)
• YX Xu, J Sun, WL Xiao, YS Liu, J Yue, LF Xue, J Deng, KQ Zhi, YL Wang MiR-4513 mediates the proliferation and apoptosis of oral squamous cell carcinoma cells via targeting CXCL17 Eur Rev Med Pharmacol Sci, 2019-05-01;23(9):3821-3828. 2019-05-01 [PMID: 31115009] (Western Blot, Human)
• Yomna Rashad, Lina Olsson, Anne Israelsson, Åke Öberg, Gudrun Lindmark, Marie-Louise Hammarström et al. Lymph node CXCL17 messenger RNA: A new prognostic biomarker for colon cancer Tumour Biol 9/11/2018 [PMID: 30198422]
• Ohlsson L, Hammarstrom ML, Lindmark G et al. Ectopic expression of the chemokine CXCL17 in colon cancer cells Br. J. Cancer 2/18/2016 [PMID: 26889977]
• Burkhardt A, Maravillas-Montero J, Carnevale C, Vilches-Cisneros N, Flores J, Hevezi P, Zlotnik A CXCL17 is a major chemotactic factor for lung macrophages. J Immunol, 2014-06-27;193(3):1468-74. 2014-06-27 [PMID: 24973458] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CXCL17
• Uniprot:
  • Q6UXB2()