CXCL1/GRO alpha/KC/CINC-1 Antibody

Product Discontinued

CXCL1/GRO alpha/KC/CINC-1 Antibody Summary

• Immunogen: Peptide with sequence C-EKMLNSDKSN corresponding to C-Terminus according to NP_001502.1.
• Epitope: C-EKMLNSDKSN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as small as the calculated size of 11.3 kDa band according to NP_001502.1.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93% and 82% identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (NBP1-52142)(1)

Publication using NBP1-52142

• Kawanishi H, Matsui Y, Ito M et al. Secreted CXCL1 is a potential mediator and marker of the tumor invasion of bladder cancer Clin Cancer Res 2008-05-01 [PMID: 18451219]

Bioinformatics

• Gene Symbol: CXCL1
• Entrez:
  • 2919(Human)
• Uniprot:
  • NP_001502.1(Human)