CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [Unconjugated]

Recombinant Human CXCL1/GRO alpha /KC/CINC‑1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GRO alpha /KC/CINC‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275). The ND50 is typically 3-15 µg/mL.

Human peripheral blood mononuclear cell (PBMCs) treated with 1 μg/mL LPS for 24 hours and3 μM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

• Reactivity: Hu, Pm
• Applications: WB, B/N, CyTOF-ready, ELISA(Cap), Flow
• Clone: 20326
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1
• Specificity: Detects human CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr)CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhMIP-1 alpha, recombinant mouse (rm)MIP-1 alpha, rmMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 3-15 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human CXCL1/GROalpha /KC/CINC-1 Biotinylated Antibody (Catalog number BAF275)
  Standard: Recombinant Human CXCL1/GROalpha /KC/CINC-1 (Catalog number 275-GR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• GRO alpha
• KC
• MGSA-alpha

Background

The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines.

The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.

Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (MAB275)(11)

Publications using MAB275

• SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018;9(1):3267. 2018 [PMID: 30111846] (Neutralization, Human)
• Y Kitawaki, Y Nakamura, F Kubota-Nak, Y Yamazaki, Y Miki, S Hata, K Ise, K Kikuchi, R Morimoto, F Satoh, H Sasano Tumor Microenvironment in Functional Adrenocortical Adenomas: Immune Cell Infiltration in Cortisol-producing Adrenocortical Adenoma Hum. Pathol., 2018;0(0):. 2018 [PMID: 29596893] (IHC-P, Human)
• EK Kim, S Moon, DK Kim, X Zhang, J Kim CXCL1 induces senescence of cancer-associated fibroblasts via autocrine loops in oral squamous cell carcinoma PLoS ONE, 2018;13(1):e0188847. 2018 [PMID: 29360827] (Neutralization, Human)
• CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017;65(4):715-729.e5. 2017 [PMID: 28212752] (ICC, Human)
• Kelvin K Tsai Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells J. Exp. Med., 2016;0(0):. 2016 [PMID: 27881732] (Neutralization, Human)
• Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke. Blood, 2012;120(18):3793-802. 2012 [PMID: 22976954] (IHC-P, Human)
• Oladipo O, Conlon S, O'Grady A The expression and prognostic impact of CXC-chemokines in stage II and III colorectal cancer epithelial and stromal tissue. Br. J. Cancer, 2011;104(3):480-7. 2011 [PMID: 21285972] (IHC-P, Human)
• Meen AJ, Oynebraten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1. J. Biol. Chem., 2011;286(4):2636-47. 2011 [PMID: 21075844] (ICC, Human)
• Moscova M, Marsh DJ, Baxter RC Protein chip discovery of secreted proteins regulated by the phosphatidylinositol 3-kinase pathway in ovarian cancer cell lines. Cancer Res., 2006;66(3):1376-83. 2006 [PMID: 16452192] (Immunodepletion, Human)
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005;175(9):6177-89. 2005 [PMID: 16237115] (Neutralization, Human)
• Hagiwara H, Mitsumata M, Yamane T Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells. Circulation, 1998;98(23):2584-90. 1998 [PMID: 9843467] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CXCL1