CXCL1/2/3/GRO Antibody (29702) [Unconjugated] - Pan Specific

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 29702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL1/2/3/GRO Antibody (29702) [Unconjugated] - Pan Specific Summary

• Immunogen: E. coli-derived recombinant human CXCL3
  Ala35-Asn107
  Accession # P19876
• Specificity: Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/2/3/GRO Antibody (29702) [Unconjugated] - Pan Specific

• CXCL1/2/3
• FSP
• GRO1
• GROa
• MGSA
• MGSA-a
• NAP-3
• SCYB1

Background

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha , GRO beta (MIP-2 alpha ) and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/2/3/GRO Antibody (MAB2761)(1)

Publication using MAB2761

• Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment Cell reports 2023-12-30 [PMID: 38232734] (Mass Cytometry, Mouse)

Bioinformatics

• Uniprot:
  • P19876()