CXADR Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXADR Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CXADR
  Leu20-Gly237
  Accession # P78310
• Specificity: Detects human CXADR in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse CXADR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXADR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXADR Antibody

• CAR10Coxsackievirus B-adenovirus receptor
• CAR4/6
• coxsackie virus and adenovirus receptor
• coxsackie virus B receptor
• coxsackievirus and adenovirus receptor
• CVB3 binding protein
• CVB3 BP
• CVB3-binding protein
• CXADR
• HCAR
• HCVADR

Background

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C‑terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows a similar expression pattern (4, 10). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (11).

1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
5. Hotta, Y. et al. Dev. Brain Res. 143:1.
6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
7. Chen, J. et al. (2006) Circ. Res. 98:923.
8. Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
9. Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
10. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
11. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXADR Antibody (AF3336)(4)

Publications using AF3336

• Azadeh Nilchian, Estelle Plant, Malgorzata M Parniewska, Ana Santiago, Aránzazu Rossignoli, Josefin Skogsberg et al. Induction of the Coxsackievirus and Adenovirus Receptor in Macrophages During the Formation of Atherosclerotic Plaques The Journal of Infectious Diseases 11/13/2020 [PMID: 32852032]
• N Mestre-Fra, N Serratrice, A Gennetier, G Devau, S Cobo, SG Trouche, P Fontès, C Zussy, P De Deurwae, S Salinas, FJ Mennechet, J Dusonchet, BL Schneider, I Saggio, V Kalatzis, MR Luquin-Piu, JM Verdier, EJ Kremer Exogenous LRRK2G2019S induces parkinsonian-like pathology in a nonhuman primate JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046008] (IHC, Primate)
• M. N. Garas, S. V. Tillib, O. V. Zubkova, V. N. Rogozhin, T. I. Ivanova, L. A. Vasilev et al. Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting Acta Naturae 8/6/2014 [PMID: 25093116]
• M. N. Garas, S. V. Tillib, O. V. Zubkova, V. N. Rogozhin, T. I. Ivanova, L. A. Vasilev et al. Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting Acta Naturae 2014-08-06 [PMID: 25093116] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CXADR
• Uniprot:
  • P78310()