CWC22 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

CWC22 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CWC22.
  
  

  Source: E. coli

  Amino Acid Sequence: RNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSRGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDP

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CWC22
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84864.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CWC22 Recombinant Protein Antigen

• CWC22 spliceosome-associated protein homolog (S. cerevisiae)
• EIF4GL
• fSAPbpre-mRNA-splicing factor CWC22 homolog
• KIAA1604NCMfunctional spliceosome-associated protein b
• Nucampholin homolog

Background

CWC22, also known as Pre-mRNA-splicing factor CWC22 homolog, consists of a long 908 amino acid isoform that is 105 kDa, and is involved in pre-mRNA splicing, though its exact function within the process is still a focus of research. Current disease research is being conducted on the relation between CWC22 and nevus, cerebral malaria, cerebritis, and malaria. The protein is linked to a RNA metabolic processing and mRNA splicing, through which it interacts with DHX15, PRPF6, EIF4A3, FAM32A, and FRG1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CWC22