Cullin 4a Antibody (JU07-33)

Western Blot: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.

Flow Cytometry: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cullin 4a Antibody (JU07-33) [NBP2-75459] - Analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.

Product Discontinued

Cullin 4a Antibody (JU07-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cullin 4a aa 710-759 / 759. (SwissProt: Q13619 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CUL4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-2000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cullin 4a Antibody (JU07-33)

• CUL4A
• CUL-4A
• Cullin 4a
• cullin-4A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUL4A