CTCF Antibody (JM10-61)

Western Blot: CTCF Antibody (JM10-61) [NBP2-67001] - Western blot analysis of CTCF on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: CTCF Antibody (JM10-61) [NBP2-67001] - Staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Flow Cytometry: CTCF Antibody (JM10-61) [NBP2-67001] - Flow cytometric analysis of CTCF was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution. Positive control: Lane 1: 293T Lane 2:MCF-7

Western Blot: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.

Western Blot: CTCF Antibody (JM10-61) [NBP2-67001] - Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western Blot: CTCF Antibody (JM10-61) [NBP2-67001] - Western blot analysis of CTCF on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: CTCF Antibody (JM10-61) [NBP2-67001] - Staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CTCF Antibody (JM10-61) [NBP2-67001] - Staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CTCF Antibody (JM10-61) [NBP2-67001] - Analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.

Product Discontinued

CTCF Antibody (JM10-61) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CTCF aa 685-727 / 727. (SwissProt: P49711 Human; SwissProt: Q61164 Mouse; SwissProt: Q9R1D1 Rat)
• Localization: Nucleoplasm, Chromosome, centromere.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CTCF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CTCF Antibody (JM10-61)

• 11 zinc finger transcriptional repressor
• 11-zinc finger protein
• 11-Zinc Finger Protein2
• CCCTC-binding factor (zinc finger protein)
• CCCTC-binding Factor
• CTCF
• CTCFL paralog
• transcriptional repressor CTCF

Background

The CTCF gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA tar

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTCF