CtBP1 Overexpression Lysate

Western Blot: CtBP1 Overexpression Lysate (Adult Normal) [NBL1-09564] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CtBP1.

• Reactivity: Hu
• Applications: WB

CtBP1 Overexpression Lysate Summary

• Description: Transient overexpression lysate of C-terminal binding protein 1 (CTBP1), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC208594 and based on accession number NM_001012614. The protein contains a C-MYC/DDK Tag.
• Gene: CTBP1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 46.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CtBP1 Overexpression Lysate

• BARS
• brefeldin A-ribosylated substrate
• CTBP
• CtBP1
• C-terminal binding protein 1
• C-terminal-binding protein 1
• EC 1.1.1
• EC 1.1.1.-
• EC 1.1.1.158
• MGC104684

Background

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (2) has an alternate in-frame exon in the 5' coding region which results in translation initiation at a downstream ATG, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CTBP1