CRTH-2/GPR44 Antibody Blocking Peptide

Western Blot: GPR44 Peptide [NBP1-76755PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.

• Reactivity: Hu
• Applications: AC
• Concentration: 0.2 mg/ml

CRTH-2/GPR44 Antibody Blocking Peptide Summary

• Description: A blocking peptide containing 18 amino acid peptide from near the amino terminus of human CRTH2.

  Source: Synthetic

  (Accession #: NP_004769)

• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: PTGDR2
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NBP1-76755.
  
  For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: N/A

Alternate Names for CRTH-2/GPR44 Antibody Blocking Peptide

• CD294 antigen
• CD294
• Chemoattractant receptor-homologous molecule expressed on Th2 cells
• CRTH2
• CRTH-2
• CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• DL1R
• DP2
• G protein-coupled receptor 44
• GPR44
• PGD2R
• PTGDR2
• putative G-protein coupled receptor 44

Background

GPR44, also called CRTH2, is a Prostanoid Receptor that binds prostaglandin D2. PGD2 signals through CRTH2 to induce Th2, eosinophil, and basophil chemotaxis in a G-alpha(i) dependent manner. CRTH2 may play a role in mast cell-mediated immune responses such as those involved in allergic reactions. This receptor is upregulated in activated T helper-2 (Th2) cells, peripheral blood basophils, and eosinophils, but not in Th1 lineage cells. It may be a useful marker for Th2 and Tc2 cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated, a 3.5-kb transcript expressed primarily in the thalamus, frontal cortex, pons, and hippocampus, and a 3.4-kb transcript expressed in fetal liver, leukocytes, and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis, and thymus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PTGDR2