CRTH-2/GPR44 Antibody

Western Blot: CRTH-2/GPR44 Antibody [NBP1-50452] - Analysis of anti-GPR44 / CRTH2 antibody with Jurkat cell lysate at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: CRTH-2/GPR44 Antibody [NBP1-50452] - Analysis of anti-GPR44 / CRTH2 antibody with human heart cells.

Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-50452] - Analysis of anti-GPR44 / CRTH2 antibody with human adrenal at concentration 5 ug/ml.

Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-50452] - Analysis of anti-GPR44 / CRTH2 antibody with human brain at concentration 5 ug/ml.

Immunohistochemistry-Paraffin: CRTH-2/GPR44 Antibody [NBP1-50452] - Analysis of anti-GPR44 / CRTH2 antibody with human adrenal at concentration 5 ug/ml.

Product Discontinued

CRTH-2/GPR44 Antibody Summary

• Immunogen: An 18 aa synthetic peptide near the N-terminus of human CRTH-2/GPR44. The immunogen is located within the first 50 aa of CRTH-2/GPR44.
• Epitope: N-Terminus
• Specificity: At least two different isoforms of CRTH-2/GPR44 are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGDR2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CRTH-2/GPR44 Antibody

• CD294 antigen
• CD294
• Chemoattractant receptor-homologous molecule expressed on Th2 cells
• CRTH2
• CRTH-2
• CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• DL1R
• DP2
• G protein-coupled receptor 44
• GPR44
• PGD2R
• PTGDR2
• putative G-protein coupled receptor 44

Background

GPCR GPR44 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated: a 3.5 kb transcript expressed primarily in the thalamus, frontal cortex, pons and hippocampus; and a 3.4 kb transcript expressed in fetal liver, leukocytes and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis and thymus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGDR2
• Entrez:
  • 11251(Human)
• Uniprot:
  • Q9Y5Y4()