CRTAM Antibody (210213) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin for 24 hours were stained with Mouse Anti-Human CRTAM APC-conjugated Monoclonal Antibody (Catalog # FAB16951A) and Mouse Anti-Human CD8a PE-conjugated Monoclonal Antibody (Catalog # FAB1509P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 210213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CRTAM Antibody (210213) [Allophycocyanin] Summary

• Additional Information: Clone 210213 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CRTAM
  Ser18-Ser286
  Accession # O95727
• Specificity: Detects human CRTAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CRTAM is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRTAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRTAM Antibody (210213) [Allophycocyanin]

• CD355 antigen
• CD355
• class I MHC restricted T cell associated molecule
• class-I MHC-restricted T cell associated molecule
• Class-I MHC-restricted T-cell-associated molecule
• CRTAM
• cytotoxic and regulatory T cell molecule
• cytotoxic and regulatory T-cell molecule

Background

CRTAM (Class I-restricted T cell-associated molecule) is a nectin family member of the immunoglobulin superfamily that is expressed by activated CD8⁺ and NK T cells (1‑4). NK activation receptor engagement, but not cytokines, also induce NK CRTAM expression (4, 5). CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum (1, 2). Human CRTAM is a 393 amino acid (aa), 80 kDa type I transmembrane glycoprotein with a 17 aa signal sequence, a 269 aa extracellular domain (ECD), a 21 aa transmembrane segment and an 84 aa cytoplasmic domain. The ECD has one V-type and one C1-type Ig-like domain, while the cytoplasmic region shows a potential class I PDZ domain (1‑5). Human CRTAM ECD shows 70%, 43% and 63% aa identity with mouse, rat and canine CRTAM ECD, respectively, but 73‑78% aa identity within the Ig-like domains. The V-type Ig-like domain mediates interaction with the corresponding domain on another nectin family member, IGSF4 (also called TSLC-1, Necl-2, Syncam or SgIGSF) (4, 5). CRTAM is a homodimer on the cell surface but does not show homotypic binding in trans (3‑5). The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3‑5). IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8⁺ cells induces increased IFN-gamma or IL-22 production (3, 4). A role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed (3‑5). IGSF4 is expressed broadly, including on epithelia, neurons, a subset of tonsillar B cells (4, 5), and a rare splenic T zone-restricted BCDA3+ dendritic cell population which interacts with CRTAM (3).

1. Kennedy, J. et al. (2000) J. Leukoc. Biol. 67:725.
2. Patino-Lopez, G. et al. (2006) J. Neuroimmunol. 171:145.
3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
4. Boles, K. S. et al. (2005) Blood 106:779.
5. Arase, N. et al. (2005) Int. Immunol. 17:1227.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTAM
• Uniprot:
  • O95727()