CrkL Antibody

Images

 
Western Blot: CrkL Antibody [NB300-884] - K562 cell lysate (35 ug protein in RIPA buffer). Primary antibody at 0.01 ug/mL, 1 hr incubation. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CrkL Antibody [NB300-884] - Paraffin embedded human thymus. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CrkL Antibody Summary

Immunogen
Peptide with sequence C-KIFDPQNPDENE corresponding to C-Terminus according to NP_005198.1.
Localization
Cell membrane
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRKL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2.5 - 3.8 ug/mL
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 37 kDa band observed in lysates of K562, Jurkat, NIH3T3, and of rat spleen (calculated MW of 33.8 kDa band according to NP_005198). IHC-P: Human thymus shows staining of cellular membranes in thymocytes.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CrkL Peptide (NB300-884PEP)
Publications
Read Publication using NB300-884.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CrkL Antibody

  • CrkL
  • crk-like protein
  • pCRKL
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
  • v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Background

CRKL is a 33kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. CRKL is a substitute of BCR ABL tyrosine kinase. In addition, CRKL has oncogenic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CrkL Antibody (NB300-884)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRKL
Entrez
Uniprot