CRISP-2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

CRISP-2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CRISP2.
  
  

  Source: E. coli

  Amino Acid Sequence: GCPDDCDKGLCTNSCQYQDLLSNCDSLKNTAGCEHELLKEKCKATCLCEN

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CRISP2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85452.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CRISP-2 Recombinant Protein Antigen

• Cancer/testis antigen 36
• CRISP2
• CRISP-2
• CRISP-2GAPDL5
• CT36TSP1
• cysteine-rich secretory protein 2
• GAPDL5
• glyceraldehyde-3-phosphate dehydrogenase-like 5
• testis specific protein 1 (probe H4-1 p3-1)
• Testis-specific protein TPX-1
• TPX1
• TPX1MGC111136
• TSP1

Background

CRISP2, or Cysteine-rich secretory protein 2, consists of a 243 amino acid isoform that is 27 kDa, and is involved in the regulation of calcium fluxation and ion channel activity during sperm capacitation. Current research is being conducted on CRISP2 and its relation to several diseases and disorders, including male infertility, atopic dermatitis, and sinusitis. The protein is not currently linked to any specific pathways or biological processes, but it interacts with PPP2R1A and PPP2R1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRISP2