CRIPTO Antibody (237603) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
237603
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

CRIPTO Antibody (237603) [Alexa Fluor® 594] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cripto
Arg26-Gln150
Accession # P51865
Specificity
Detects mouse Cripto in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ART, recombinant mouse (rm) ART, rmArtemin, recombinant drosophila DPP, rhGDNF, recombinant rat (rr) GDNF, rhLAP, rhLatent TGF-beta 1, rhTGF-beta 2, rhLefty-A, rmLefty-1, rrMIS, rhNeurturin, rmNodal, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TDGF1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRIPTO Antibody (237603) [Alexa Fluor® 594]

  • CFC-2
  • CR
  • Cripto
  • Cripto-1 growth factor
  • Cripto-1
  • CRIPTOCRGF
  • Epidermal growth factor-like cripto protein CR1
  • TDGF1
  • TDGF-1
  • teratocarcinoma-derived growth factor 1

Background

Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved cysteine‑rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the
C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Mouse Cripto shares 66% and 34% amino acid identity with human Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3). Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2). Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to an Act RIIB‑ALK4‑Cripto‑Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Work has shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7).

  1. Rosa, F.M. (2002) Science’s STKE http://stke.sciencemag.org/.
  2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
  3. Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439.
  4. Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199.
  5. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
  6. Cheng, S. et al. (2003) Genes & Dev. 17:31.
  7. Bianco, C. et al. (2003) Cancer Research 63:1192.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1538U
Alexa Fluor 405 FAB1538V
Alexa Fluor 488 FAB1538G
Alexa Fluor 594 FAB1538T
Alexa Fluor 647 FAB1538R
Alexa Fluor 700 FAB1538N
Alexa Fluor 750 FAB1538S
Allophycocyanin FAB1538A

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Bioinformatics

Gene Symbol TDGF1
Uniprot
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