CRHR1/CRF1 Antibody (343919)

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 343919
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CRHR1/CRF1 Antibody (343919) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CRHR-1
  Ser24-Val415
  Accession # NP_004373
• Specificity: Detects human CRHR‑1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRHR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRHR1/CRF1 Antibody (343919)

• CHR-R1
• corticotropin releasing hormone receptor 1
• corticotropin-releasing factor receptor 1
• corticotropin-releasing factor type 1 receptor
• Corticotropin-releasing hormone receptor 1
• CRF1
• CRFR
• CRF-R
• CRFR1
• CRF-R1
• CRF-R-1
• CRFR-1
• CRHR1
• CRH-R-1
• CRHR1f
• CRH-R1h
• CRHR1L
• CRHRCRH-R1
• seven transmembrane helix receptor

Background

Corticotrophin-Releasing Hormone Receptor 1 (CRHR-1), also known as CRFR-1, is a 7TM protein that belongs to the G protein-coupled receptor family 2. CRHR-1 is expressed in the brain and adrenal gland where it mediates corticotropin-induced effects on anxiety, depression, and stress-associated pathologies. The immunogen for this antibody corresponds to one of the four alternately spliced forms and has a 29 amino acid deletion in the first cytoplasmic domain. Human CRHR-1 shares 97% amino acid sequence identity with mouse and rat CRHR-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRHR1/CRF1 Antibody (MAB3930)(2)

Publications using MAB3930

• Y Isozaki, K Tsumoto, M Tomita Conformation-specific monoclonal antibodies recognizing the native structure of G protein-coupled receptor (GPCR) International immunopharmacology, 2021-06-25;98(0):107872. 2021-06-25 [PMID: 34182241] (IHC, Chinese Hamster)
• Shumsuzzaman Khan, Xinjiang Lu, Qingyao Huang, Jiawei Tang, Jian Weng, Zhi Yang et al. IGFBP2 Plays an Essential Role in Cognitive Development during Early Life Advanced Science 2019-12-01 [PMID: 31832311] (Immunocytochemistry, Mouse)

Bioinformatics

• Gene Symbol: CRHR1
• Uniprot:
  • NP_004373()