CRELD2 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRELD2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CRELD2
  Ser23-Leu350
  Accession # Q9CYA0
• Specificity: Detects mouse CRELD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CRELD2 is observed and less than 1% cross-reactivity with recombinant mouse CRELD1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRELD2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRELD2 Antibody

• CRELD2
• cysteine-rich with EGF-like domain protein 2
• cysteine-rich with EGF-like domains 2
• DKFZp667O055
• MGC11256

Background

CRELD2 is a ubiquitously expressed protein that is the second member of the CRELD family of matricellular proteins. Mouse CRELD2 is synthesized as a 350 amino acid precursor protein with a signal peptide, a highly conserved domain rich in glutamic acid and tryptophan (WE), and EGF-like repeats. Human CRELD2 has been shown to interact with the alpha 4 subunit of the nicotinic acetylcholine receptor and function as a specific regulator of receptor expression. Mouse CRELD2 shares 77% amino acid sequence homology with the human CRELD2 alpha isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRELD2 Antibody (AF3686)(2)

Publications using AF3686

• KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease PLoS Genet., 2019;15(7):e1008215. 2019 [PMID: 31260448] (IHC, Mouse)
• Y Kim, SJ Park, SR Manson, CA Molina, K Kidd, H Thiessen-P, RJ Perry, H Liapis, S Kmoch, CR Parikh, AJ Bleyer, YM Chen Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease JCI Insight, 2017;2(23):. 2017 [PMID: 29212948] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CRELD2
• Uniprot:
  • Q9CYA0(Mouse)