CRELD1 Antibody

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CRELD1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse CRELD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4116) at 10 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CRELD1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CRELD1
Gln30-Glu362
Accession # Q91XD7
Specificity
Detects mouse CRELD1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CRELD1 is observed and less than 5% cross-reactivity with recombinant mouse CRELD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRELD1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRELD1 Antibody

  • atrioventricular septal defect 2
  • AVSD2
  • CIRRIN
  • CRELD1
  • cysteine-rich with EGF-like domain protein 1
  • cysteine-rich with EGF-like domains 1
  • DKFZp566D213

Background

CRELD1 (cysteine-rich with EGF-like domain protein 1) is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain (ECD) with two EGF-like repeats that flank two consecutive furin-like repeats, two‑transmembrane domains separated by one cytoplasmic residue, and a short extracellular C-terminal domain. CRELD1 null-mouse fails to develop a normal heart and missense mutations in human CRELD1 have also been associated with atrioventricular septal defects. The N-terminal ECD of mouse CRELD1 shares 97% and 92% aa sequence identity with the ECD of rat and human CRELD1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CRELD1
Uniprot