CRELD1 Antibody

CRELD1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse CRELD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4116) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing heart and is shown at both low (lower panel) and high magnification (upper panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRELD1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CRELD1
  Gln30-Glu362
  Accession # Q91XD7
• Specificity: Detects mouse CRELD1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CRELD1 is observed and less than 5% cross-reactivity with recombinant mouse CRELD2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRELD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRELD1 Antibody

• atrioventricular septal defect 2
• AVSD2
• CIRRIN
• CRELD1
• cysteine-rich with EGF-like domain protein 1
• cysteine-rich with EGF-like domains 1
• DKFZp566D213

Background

CRELD1 (cysteine-rich with EGF-like domain protein 1) is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain (ECD) with two EGF-like repeats that flank two consecutive furin-like repeats, two‑transmembrane domains separated by one cytoplasmic residue, and a short extracellular C-terminal domain. CRELD1 null-mouse fails to develop a normal heart and missense mutations in human CRELD1 have also been associated with atrioventricular septal defects. The N-terminal ECD of mouse CRELD1 shares 97% and 92% aa sequence identity with the ECD of rat and human CRELD1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRELD1 Antibody (AF4116)(1)

Publication using AF4116

• Jennifer K. Redig, Gameil T. Fouad, Darcie Babcock, Benjamin Reshey, Eleanor Feingold, Roger H. Reeves et al. Allelic Interaction between CRELD1 and VEGFA in the Pathogenesis of Cardiac Atrioventricular Septal Defects AIMS Genetics 2014-05-01 [PMID: 25328912]

Bioinformatics

• Gene Symbol: CRELD1
• Uniprot:
  • Q91XD7()