GRK5 Antibody [Unconjugated]

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat‑2 rat embryonic fibroblast cell line, and BaF3 mouse pro‑B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRK5 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GRK5 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GRK5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GRK5
  Pro463-Ser590
  Accession # P34947
• Specificity: Detects endogenous human, mouse, and rat GRK5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRK5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRK5 Antibody [Unconjugated]

• EC 2.7.11
• EC 2.7.11.16
• FLJ39780
• G protein-coupled receptor kinase 5
• G protein-coupled receptor kinase GRK5
• GPRK5
• GPRK5FP2025
• GRK5

Background

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRK5 Antibody (AF4539)(4)

Publications using AF4539

• NK Todd, Y Huang, JY Lee, P Doruker, JM Krieger, R Salisbury, M MacDonald, I Bahar, A Thathiah GPCR kinases generate an APH1A phosphorylation barcode to regulate amyloid-beta generation Oncogene, 2022;40(3):111110. 2022 [PMID: 35858570] (Western Blot, Human)
• TC Møller, MF Pedersen, JR van Senten, SD Seiersen, JM Mathiesen, M Bouvier, H Bräuner-Os Dissecting the roles of GRK2 and GRK3 in ?-opioid receptor internalization and &beta-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells Sci Rep, 2020;10(1):17395. 2020 [PMID: 33060647] (Western Blot, Human)
• Bychkov , Evgeny, Zurkovsky , Lilia, Garret , Mika B, Ahmed , Mohamed, Gurevich , Eugenia Distinct cellular and subcellular distributions of G protein-coupled receptor kinase and arrestin isoforms in the striatum. PLoS ONE, 2012;7(11):e48912. 2012 [PMID: 23139825] (Western Blot, Human)
• Chen Y, Wang F, Long H GRK5 promotes F-actin bundling and targets bundles to membrane structures to control neuronal morphogenesis. J. Cell Biol., 2011;194(6):905-20. 2011 [PMID: 21930777] (ICC, Mouse)

Review for GRK5 Antibody (AF4539) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous - WB Human 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23139825
• Species: Human

Bioinformatics

• Gene Symbol: GRK5
• Uniprot:
  • P34947()