GRK5 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat‑2 rat embryonic fibroblast cell line, and BaF3 mouse pro‑B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

GRK5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947
Specificity
Detects endogenous human, mouse, and rat GRK5 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRK5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Knockout Validated
Reviewed Applications
Read 1 Review rated 4
using
AF4539 in the following applications:

Publications
Read Publications using
AF4539 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRK5 Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.16
  • FLJ39780
  • G protein-coupled receptor kinase 5
  • G protein-coupled receptor kinase GRK5
  • GPRK5
  • GPRK5FP2025
  • GRK5

Background

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRK5 Antibody (AF4539)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(1)
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Filter By Species
Human
(1)
Mouse
(1)
All Species

Review for GRK5 Antibody (AF4539) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF4539:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23139825
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23139825

Details

Detection NotesSee PMID 23139825

Comments

CommentsPublished: See PMID 23139825Independent review from antibodyresource.com

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FAQs for GRK5 Antibody (AF4539) (0)

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Secondary Antibodies

 

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Recent Reviews

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4
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1
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Anonymous
01/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol GRK5
Uniprot