CREB Antibody (JB25-40)

Western Blot: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of CREB [p Ser133] on THP-1 cell lysates with Rabbit anti-Phospho-Creb (S133) antibody at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 35 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: CREB Antibody (JB25-40) [NBP2-75447] - Staining Phospho-Creb (S133) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CREB [p Ser133] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of HUVEC cells with Phospho-Creb (S133) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of Phospho-Creb (S133) on human colon tissue lysates using anti-Phospho-Creb (S133) antibody at 1/500 dilution.

Western Blot: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of Phospho-Creb (S133) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Creb (S133) antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 35 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: CREB Antibody (JB25-40) [NBP2-75447] - Staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded human spleen tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Phospho-Creb (S133) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: CREB Antibody (JB25-40) [NBP2-75447] - Analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-CREB [p Ser133] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

CREB Antibody (JB25-40) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB. (SwissProt: P16220 Human; SwissProt: Q01147 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CREB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CREB Antibody (JB25-40)

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• transactivator protein

Background

CREB (cAMP responsive element binding protein 1) stimulates transcription. CREB is involved in learning and memory. This protein is known to have interactions with HIST1H3B, HIST1h3A, HIST1H3C, HIST1H3D and HIST1H3E. CREB has been studied in relation to several diseases and disorders including Rubinstein-Taybi syndrome, Alzheimer's disease, leukemia and brain disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CREB Antibody (NBP2-75447). (Showing 1 - 1 of 1 FAQ).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Bioinformatics

• Gene Symbol: CREB1