CREB Antibody (82B514.2)

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Western Blot: CREB Antibody (82B514.2) [NB100-56525] - Analysis of CREB in HeLa cell lysate using this antibody at 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
82B514.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CREB Antibody (82B514.2) Summary

Immunogen
A portion of amino acids 100-150 of human CREB was used as the immunogen.
Specificity
In HeLa, a 43 kDa band is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CREB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
Control
CREB Overexpression Lysate
HeLa Whole Cell Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CREB Antibody (82B514.2)

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • transactivator protein

Background

The transcription factor cAMP response element binding (CREB) binds the palindromic octanucleotide TGACGTCA. Members of ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly conserved leucine zipper dimerization and basic DNA binding domains at the COOH terminal but are highly divergent in their amino terminal domains. CREB-1 is phosphorylated at Ser133, a key regulatory site controlling transcriptional activity, by many different kinases including protein kinase A, the calmodulin-dependent protein kinase CaMKII, p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. Phosphorylation allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CREB Antibody (NB100-56525). (Showing 1 - 1 of 1 FAQ).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP2-23646

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Blogs on CREB.

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By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Bioinformatics

Gene Symbol CREB1
Entrez
Uniprot
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