CREB Antibody (82B514.2)

Western Blot: CREB Antibody (82B514.2) [NB100-56525] - Analysis of CREB in HeLa cell lysate using this antibody at 2 ug/ml.

Product Discontinued

CREB Antibody (82B514.2) Summary

• Immunogen: A portion of amino acids 100-150 of human CREB was used as the immunogen.
• Specificity: In HeLa, a 43 kDa band is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CREB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/ml
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CREB Antibody (82B514.2)

• active transcription factor CREB
• cAMP responsive element binding protein 1
• cAMP-response element-binding protein-1
• cAMP-responsive element-binding protein 1
• CREB
• CREB1
• CREB-1
• cyclic AMP-responsive element-binding protein 1
• MGC9284
• transactivator protein

Background

The transcription factor cAMP response element binding (CREB) binds the palindromic octanucleotide TGACGTCA. Members of ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly conserved leucine zipper dimerization and basic DNA binding domains at the COOH terminal but are highly divergent in their amino terminal domains. CREB-1 is phosphorylated at Ser133, a key regulatory site controlling transcriptional activity, by many different kinases including protein kinase A, the calmodulin-dependent protein kinase CaMKII, p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. Phosphorylation allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CREB Antibody (NB100-56525). (Showing 1 - 1 of 1 FAQ).

1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
   • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Bioinformatics

• Gene Symbol: CREB1
• Entrez:
  • 1385(Human)
• Uniprot:
  • NP_604391(Human)