Creatine kinase MT 1B Antibody - BSA Free

Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543] - Sample(30 ug whole cell lysate) A:H1299 B:HeLa S310% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: Creatine kinase MT 1B Antibody [NBP1-31543] - Paraffin-embedded A549 xenograft, using antibody at 1:300 dilution.

Immunohistochemistry: Creatine kinase MT 1B Antibody [NBP1-31543] - Creatine kinase MT 1B antibody detects Creatine kinase MT 1B protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. Creatine kinase MT 1B antibody (NBP1-31543) dilution: 1:100.

• Reactivity: Hu, Ze, Rt, Bv, Rb
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Creatine kinase MT 1B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT 1B. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane, Peripheral membrane protein, Intermembrane side
• Predicted Species: Rat (98%), Bovine (98%), Rabbit (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKMT1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Creatine kinase MT 1B Antibody - BSA Free

• Acidic-Type Mitochondrial Creatine Kinase
• CKMT
• CKMT1
• CKMT1B
• Creatine Kinase U-Type, Mitochondrial
• Creatine Kinase, Mitochondrial 1 (Ubiquitous)
• Creatine Kinase, Mitochondrial 1B
• EC 2.7.3.2
• Mia-CK
• Ubiquitous Mitochondrial Creatine Kinase
• UMTCK
• U-MtCK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CKMT1B