Creatine kinase MT 1B Antibody

Images

 
Western Blot: Creatine kinase MT 1B Antibody [NBP1-31543] - Sample(30 ug whole cell lysate) A:H1299 B:HeLa S310% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: Creatine kinase MT 1B Antibody [NBP1-31543] - Paraffin-embedded A549 xenograft, using antibody at 1:300 dilution.

Product Details

Summary
Reactivity Hu, Rt, Bv, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Creatine kinase MT 1B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Creatine kinase 1B. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane, Peripheral membrane protein, Intermembrane side
Predicted Species
Rat (98%), Bovine (98%), Rabbit (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CKMT1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Creatine kinase MT 1B Antibody

  • Acidic-Type Mitochondrial Creatine Kinase
  • CKMT
  • CKMT1
  • CKMT1B
  • Creatine Kinase U-Type, Mitochondrial
  • Creatine Kinase, Mitochondrial 1 (Ubiquitous)
  • Creatine Kinase, Mitochondrial 1B
  • EC 2.7.3.2
  • Mia-CK
  • Ubiquitous Mitochondrial Creatine Kinase
  • UMTCK
  • U-MtCK

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CKMT1B