Creatine Kinase BB Antibody (JB78-34)

Western Blot: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Creatine Kinase B Antibody (JB78-34) [NBP2-75446] - Analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution.Positive control: Lane 1: Mouse brain Lane 2: Mouse brain Lane 3: Rat brain Lane 4: 293

Immunocytochemistry/Immunofluorescence: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Staining Creatine kinase B type in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Analysis of paraffin-embedded rat brain tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.

Flow Cytometry: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Analysis of 293T cells with Creatine kinase B type antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Analysis of paraffin-embedded human prostate tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (JB78-34) [NBP2-75446] - Analysis of paraffin-embedded mouse colon tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.

Product Discontinued

Creatine Kinase BB Antibody (JB78-34) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Creatine Kinase BB aa 27-118 / 381. (SwissProt: P12277 Human; SwissProt: Q04447 Mouse; SwissProt: P07335 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CKB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Creatine Kinase BB Antibody (JB78-34)

• B-CK
• CKB
• Creatine Kinase BB
• Creatine Kinase Isoenzyme

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKB