Creatine Kinase BB Antibody (969409) [Unconjugated]

Western blot shows lysates of mouse and rat brain (cerebellum) tissue, human brain (motor cortex and hippocampus) tissue, and human prostate tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/ Mouse/Rat Creatine Kinase BB Monoclonal Antibody (Catalog # MAB9076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase BB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Creatine Kinase BB was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat Creatine Kinase BB Monoclonal Antibody (Catalog # MAB9076) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 969409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Creatine Kinase BB Antibody (969409) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Creatine Kinase BB
  Pro2-Lys381
  Accession # P12277
• Specificity: Detects human Creatine Kinase BB in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Creatine Kinase BB Antibody (969409) [Unconjugated]

• B-CK
• CKB
• Creatine Kinase BB
• Creatine Kinase Isoenzyme

Background

Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase B (CKB), can form homodimer (BB type), and heterodimer (MB type) with creatine kinase M (CKM). MB type is found in myocardium, and homodimer BB type is found in many tissues, especially brain. The recombinant human CKB is in the BB type and its activity is measured using a phosphatase-coupled method (4).

1. Trask R.V. et al. (1988) J. Biol. Chem. 263:17142.
2. Wallimann, T. et al. (1992) Biochem, J. 281:21-40.
3. Wallimann, T. (1994) Curr. Biol. 4:42.
4. Wu, Z.L. (2011) PLoS One 6:e23172.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Creatine Kinase BB Antibody (MAB9076)(1)

Publication using MAB9076

• PD Hamilton, SL Bozeman, UP Andley Creatine kinase/&alpha-crystallin interaction functions in cataract development Biochem Biophys Rep, 2020-02-29;22(0):100748. 2020-02-29 [PMID: 32154391] (Western Blot, Mouse)

Bioinformatics

• Uniprot:
  • P12277()