CRB3 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185] - Analysis in human kidney and cerebral cortex tissues using NBP1-81185 antibody. Corresponding CRB3 RNA-seq data are presented for ...read more
Immunocytochemistry/ Immunofluorescence: CRB3 Antibody [NBP1-81185] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: CRB3 Antibody [NBP1-81185] - Staining of human duodenum shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CRB3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VRKLREKRQTEGTYRPSSEEQFSHAAEARAPQDSKETVQGCLPI
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation
  • Western Blot
Application Notes
Use in WB, IP reported in scientific literature (PMID:34404733). ICC/IF reported in scientific literature (PMID: 26493500). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CRB3 Protein (NBP1-81185PEP)
Publications
Read Publications using
NBP1-81185 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26493500). Mouse reactivity reported in scientific literature (PMID: 29155075).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CRB3 Antibody

  • crumbs 3
  • crumbs homolog 3 (Drosophila)
  • crumbs protein homolog 3
  • MGC17303

Background

The CRB3 gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding dif

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRB3 Antibody (NBP1-81185)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC-P
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRB3 Antibody (NBP1-81185). (Showing 1 - 2 of 2 FAQs).

  1. Can you please tell me if this antibody works for mouse?
    • In regards to your inquiry, we have not yet tested this antibody on mouse samples. I ran an alignment against the antigen sequence (VRKLREKRQTEGTYRPSSEEQFSHAAEARAPQDSKETVQGCLPI) with the mouse information on UniProt and found that it has very high similarity with Isoform 2. There is a chance this will work in mouse samples. If you would like to try this antibody in mouse samples, you can qualify for our Innovators Reward Program.
  2. I am planning to use CRB3 antibody (NBP1-81185) for my bovine samples for IF. Has the antibody ever been tested with bovine samples and what is the sequence indenty of the epitope sequence with respect to bovine species?
    • Our product NBP1-81185 has not yet been tested for detection of the bovine protein. I was also not able to find the bovine CRB3 protein to run an alignment against, so I do not have any homology data available.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CRB3 Antibody (NBP1-81185)

Discover related pathways, diseases and genes to CRB3 Antibody (NBP1-81185). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol CRB3
Entrez
Uniprot