CRACC/SLAMF7 Antibody (520914) [Unconjugated]

Mouse Splenocytes were stained with Rat Anti-Mouse CRACC/SLAMF7 Monoclonal Antibody (Catalog # MAB46281, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 520914
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRACC/SLAMF7 Antibody (520914) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CRACC/SLAMF7
  Ala22-Gly224
  Accession # Q8BHK6
• Specificity: Detects mouse CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CRACC is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SLAMF7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRACC/SLAMF7 Antibody (520914) [Unconjugated]

• 19A
• CD2 subset 1
• CD2-like receptor activating cytotoxic cells
• CD319 antigen
• CD319
• CRACC
• CRACCCD2-like receptor-activating cytotoxic cells
• CS119A24 protein
• Membrane protein FOAP-12
• novel LY9 (lymphocyte antigen 9) like protein
• Novel Ly9
• Protein 19A
• SLAM family member 7
• SLAMF7

Background

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig‑like V-set domain and one Ig‑like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%‑35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8⁺ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4‑7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).

1. Veillette, A. (2006) Immunol. Rev. 214:22.
2. Tovar, V. et al. (2002) Immunogenetics 54:394.
3. Murphy, J.J. et al. (2002) Biochem. J. 361:431.
4. Bouchon, A. et al. (2001) J. Immunol. 167:5517.
5. Lee, J.K. et al. (2007) J. Immunol. 179:4672.
6. Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
7. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
8. Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRACC/SLAMF7 Antibody (MAB46281)(1)

Publication using MAB46281

• A combination of an anti-SLAMF6 antibody and ibrutinib efficiently abrogates expansion of chronic lymphocytic leukemia cells Oncotarget, 2016-05-03;7(18):26346-60. 2016-05-03 [PMID: 27029059] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SLAMF7
• Uniprot:
  • Q8BHK6()