CPSF2 Antibody - BSA Free

Western Blot: CPSF2 Antibody [NBP2-15989] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000

Immunohistochemistry: CPSF2 Antibody [NBP2-15989] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using CPSF2 antibody [N1N3] (NBP2-15989) at 1:200 dilution.

CPSF2 antibody [N1N3] detects CPSF2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CPSF2 protein stained by CPSF2 antibody [N1N3] (NBP2-15989) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Ze, Mu, Rt, Bv, Ch, Pm, Xp, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CPSF2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CPSF2 Antibody - BSA Free (NBP2-15989) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CPSF2. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (100%), Xenopus (98%), Monkey (100%), Zebrafish (96%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CPSF2 Antibody - BSA Free

• cleavage and polyadenylation specific factor 2, 100kDa
• Cleavage and polyadenylation specificity factor 100 kDa subunit
• cleavage and polyadenylation specificity factor subunit 2
• CPSF 100 kDa subunit
• CPSF 100kDa subunit
• CPSF100
• KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Background

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CPSF2
• Uniprot:
  • Q9P2I0(Human)