CPSF2 Antibody

Images

 
Western Blot: CPSF2 Antibody [NB100-79823] - Detection of CPSF2 from Hela whole cell lysate (5, 15, 50 microgram), 293T (T; 50 microgram) and mouse NIH3T3 (M; 50 microgram). CPSF100 was also immunoprecipitated by rabbit ...read more
Immunohistochemistry: CPSF2 Antibody [NB100-79823] - FFPE section of mouse teratoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823] - Human pancreatic islet cell tumor (left) and mouse teratoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry: CPSF2 Antibody [NB100-79823] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CPSF2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human cleavage and polyadenylation specific factor 2, 100kDa using the numbering given in entry NP_059133.1 (GeneID 53981).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPSF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CPSF2 Lysate (NBL1-09446)
Publications
Read Publications using
NB100-79823 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPSF2 Antibody

  • cleavage and polyadenylation specific factor 2, 100kDa
  • Cleavage and polyadenylation specificity factor 100 kDa subunit
  • cleavage and polyadenylation specificity factor subunit 2
  • CPSF 100 kDa subunit
  • CPSF 100kDa subunit
  • CPSF100
  • KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Background

CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3'-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPSF2 Antibody (NB100-79823)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for CPSF2 Antibody (NB100-79823) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CPSF2 Antibody (NB100-79823)

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Bioinformatics

Gene Symbol CPSF2
Entrez
Uniprot