CPSF2 Antibody

Western Blot: CPSF2 Antibody [NB100-79823] - Detection of CPSF2 from Hela whole cell lysate (5, 15, 50 microgram), 293T (T; 50 microgram) and mouse NIH3T3 (M; 50 microgram). CPSF100 was also immunoprecipitated by rabbit anti-CPSF100 antibodies NB100-79824 and NB100-79825.

Immunohistochemistry-Paraffin: CPSF2 Antibody [NB100-79823] - Human pancreatic islet cell tumor (left) and mouse teratoma (right). Antibody used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CPSF2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human cleavage and polyadenylation specific factor 2, 100kDa using the numbering given in entry NP_059133.1 (GeneID 53981).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Chip, WB reactivity reported in (PMID: 25274738).
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CPSF2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CPSF2 Antibody

• cleavage and polyadenylation specific factor 2, 100kDa
• Cleavage and polyadenylation specificity factor 100 kDa subunit
• cleavage and polyadenylation specificity factor subunit 2
• CPSF 100 kDa subunit
• CPSF 100kDa subunit
• CPSF100
• KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Background

CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3'-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPSF2 Antibody (NB100-79823)(3)

Publications using NB100-79823

• Pieraccioli M, Caggiano C, Mignini L et al. The transcriptional terminator XRN2 and the RNA-binding protein Sam68 link alternative polyadenylation to cell cycle progression in prostate cancer Nature structural & molecular biology 2022-11-01 [PMID: 36344846] (WB, Human)
• Tran DD, Saran S, Williamson AJ et al. THOC5 controls 3'end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100). Nucleic Acids Res. 2014-10-01 [PMID: 25274738] (WB, Chemotaxis, Mouse)
  
  Details:
  CPSF2 antibody used for ChIP on ERT2Cre THOC5 (flox/flox) MEF cells. Antibody also used for WB on Mouse NIH3T3 cells were transfected with CPSF100-specific siRNA and control siRNA and cell lysates .
• Sagawa F, Ibrahim H, Morrison AL et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 2011-08-01 [PMID: 21822216]

Bioinformatics

• Gene Symbol: CPSF2
• Entrez:
  • 53981(Human)
  • 51786(Mouse)
• Uniprot:
  • Q9P2I0(Human)