CPNE1 Antibody

Western Blot: CPNE1 Antibody [NBP1-51952] - (0.1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CPNE1 Antibody Summary

• Description: Novus Biologicals Goat CPNE1 Antibody (NBP1-51952) is a polyclonal antibody validated for use in WB and ELISA. Anti-CPNE1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-APLKPLPPSAKDPA corresponding to C-Terminus according to NP_690902.1, NP_003906.2.
• Epitope: C-APLKPLPPSAKDPA
• Specificity: This antibody is expected to recognize isoform a and b (NP_003906.2; NP_690902.1). Reported variants represent identical protein: NP_690905.1, NP_690902.1, NP_690903.1, NP_690906.1, NP_690904.1, NP_690907.1, NP_690908.1.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CPNE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human skin lysates (calculated MW of 59.1 kDa band according to NP_690902.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CPNE1 Antibody

• copine ICPN1COPN1
• copine-1
• MGC1142

Background

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Sequence analysis identified multiple alternatively spliced variants in the 5' UTR. All variants encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPNE1 Antibody (NBP1-51952)(1)

Publication using NBP1-51952

• Zhang Y, Gao Q, Duan S et al. Upregulation of Copine1 in trabecular meshwork cells of POAG patients: a membrane proteomics approach. Mol Vis 2008-05-30 [PMID: 18523666]

Bioinformatics

• Gene Symbol: CPNE1
• Entrez:
  • 8904(Human)
• Uniprot:
  • NP_690902.1(Human)