COX4 Antibody (854) - Azide and BSA Free

Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human colon cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000

Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug rat muscle lysate/extract 15 % SDS-PAGE COX4 antibody [854] dilution: 1:1000.

Western Blot: COX4 Antibody (854) [NBP2-43540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [854]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: COX4 Antibody (854) [NBP2-43540] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human ovarian cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.

Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-43540) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Pm, RM
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: 854
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

COX4 Antibody (854) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse COX4 Antibody (854) - Azide and BSA Free (NBP2-43540) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-COX4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
• Predicted Species: Chimpanzee (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: COX4I1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 6 ug/ml
  • Western Blot 1:500-1:3000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Titrated to saturation using various models, separated by Size, antibody dilution of 6 ug/mL
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for COX4 Antibody (854) - Azide and BSA Free

• COX IV-1
• COX4
• COX4-1
• COX4COXIV
• COX4I2
• Cytochrome c oxidase polypeptide IV
• cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
• MGC72016

Background

COX IV (cytochrome c oxidase subunit IV isoform 1) plays a pivotal role in respiratory function and assembly of the enzyme complex. This protein is known to have interactions with MT-CO2, MCL1, TMBIM4, MT-CO1 and TH1L. COX IV has been studied in relation to anemia, Huntington's disease, Alzheimer's disease, neuronitis, pancreatitis and several other diseases and disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX4 Antibody (NBP2-43540)(1)

Publication using NBP2-43540

• Howell Mc, Green R, Khalil R et Al. Lung cancer cells survive epidermal growth factor receptor tyrosine kinase inhibitor exposure through upregulation of cholesterol synthesis FASEB Bioadv 2020-02-01 [PMID: 32123859] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: COX4I1