COX-2 Antibody

Images

 
Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868] - 10x image of mouse lung tissue expressing cox-2. Secondary antibody: Alexa Fluor 546 (red), counterstain: DAPI (blue). Goat anti-mouse cox-2 immunofluorescence ...read more
Flow Cytometry: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG ...read more
Immunocytochemistry/ Immunofluorescence: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COX-2 Antibody Summary

Immunogen
Peptide with sequence C-NPTVLLKERSTEL corresponding to C-Terminus according to NP_000954.1.
Localization
Microsomal membrane
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTGS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3ug/ml
  • Flow Cytometry 10ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx 75-80 kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69 kDa according to NP_000954.1).
Control
COX-2 Lysate (NBP2-66242)
Reviewed Applications
Read 1 Review rated 5
using
NB100-868 in the following applications:

Publications
Read Publications using
NB100-868 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected from sequence similarity: Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 24854039).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COX-2 Antibody

  • COX2
  • COX-2
  • COX2cyclooxygenase 2b
  • cyclooxygenase-2
  • EC 1.14.99
  • EC 1.14.99.1
  • GRIPGHS
  • hCox-2
  • PGG/HS
  • PGH synthase 2
  • PGHS-2
  • PHS II
  • PHS-2
  • PHS-II
  • prostaglandin G/H synthase 2
  • prostaglandin G/H synthase and cyclooxygenase
  • Prostaglandin H2 synthase 2
  • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
  • Prostaglandin-endoperoxide synthase 2
  • PTGS2

Background

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COX-2 Antibody (NB100-868)(4)

Review for COX-2 Antibody (NB100-868) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-868:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin COX-2 NB100-868
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reviewed by:
tyler beach
IHC-P Mouse 05/09/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult Mouse Lung Tissue
SpeciesMouse
Lotc2e260417

Comments

CommentsGoat anti mouse cox 2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections-
Antigen retrieval in citrate buffer (95 C, 20 min) followed by permeabilization with 0.2% Triton x-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Counter-stained with DAPI (blue)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COX-2 Antibody (NB100-868). (Showing 1 - 1 of 1 FAQs).

  1. I am a student researching COX-2 expression in felines and am looking for a suitable primary antibody to use in IHC. To your knowledge, have any of your COX-2 antibodies been used successfully with feline COX-2? If yes, I would be very interested in learning more. If not, would it be possible to run a sequence homology of feline COX-2 (GenBank ABM65700.1, accession EF036473.1) against your available COX-2 antibodies to determine the overlap and likelihood of cross-reactivity?
    • We have not tested any of these antibodies for their reactivity in felines. However, we do have a number of antibodies that look like they may cross-react with feline. NBP1-85499 recognizes a region that is about 90-91% similar to feline COX2. NBP2-04015 recognizes a region in humans that is 94% similar to feline COX2. NB100-868 recognizes a region in humans at the far C-terminal 12 amino acids that is 100% identical to feline COX2. NBP1-19354 recognizes a region that is also about 95% similar to feline COX2. Only NBP1-85499 and NBP1-19354 have been tested for use in IHC. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
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3
0
2
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1
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tyler beach
05/09/2018
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol PTGS2
Entrez
Uniprot